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Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms.
The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available.
Includes a variety of analytical methods and how to apply the methods using examples from specific case studies
Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides
Contains easy-to-follow protocols with detailed explanations for self-guidance
Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment
Grad students, post docs, researchers in plant science
Secondary audience: Grad students, post docs, researchers in biochemistry, molecular biology, microbiology, and environmental science.
Part I.General Analytical Methods
Chapter 1.Determination of carbohydrates metabolism molecules
Part II. Purification and analysis of proteins and carbohydrates
Chapter 2.Preparation of protein extracts
Chapter 3.Protein and carbohydrate separation and purification
Chapter 4.Measurement of enzyme activity
Chapter 5.General introduction to Mass Spectrometry and Nuclear Magnetic Resonance
Part III.Case studies
Chapter 14.Sugar phosphates
Chapter 15.Nucleotide sugars